Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 53 12 2024 12 08 2789 2801 Pankaj Suden School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India Inderpal Singh School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India Chitrakshi Chopra School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India Malvika Mehta School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India Ratna Chandra School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India Indu Bhushan School of Biotechnology, Shri Mata Vaishno Devi University, Katra, India 2023 11 03 2024 02 12 Background: Nipah virus is a pathogenic virus of ruinous zoonotic potential with inflated rate of mortality in humans. Methods: Considering the emerging threat of this pandemic virus, the present investigation amid to design vaccine by using the bioinformatics tools such as host and virus codon usage analysis, CD8+ peptide prediction, immunogenicity/allergenicity/toxicity, MHC-I allele binding prediction and subsequent population coverage and MHC-I-peptide docking analysis. Results: In this study (conducted in 2022 at School of Biotechnology, Katra, India), a set of 11 peptides of the structural proteins of Nipah Virus were predicted and recognized by the set of MHC-I alleles that are expressed in 92% of the global human population. Conclusion: The strong interactions between these peptides and the MHC-I protein suggest them as strong peptide candidates for the development of vaccine against Nipah Virus. https://ijph.tums.ac.ir/index.php/ijph/article/view/33645 https://ijph.tums.ac.ir/index.php/ijph/article/download/33645/8425