Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 39 1 2010 03 15 85 91 T Mohammadian M Doosti M Paknejad F Siavoshi S Massarrat 2015 10 03 Background: Alkyl hydroperoxide reductase (AhpC) of Helicobacter pylori is considered as a diagnostic antigen. There­fore, this antigen can be used to detect H. pylori infection by stool immunoassays such as ELISA. The aim of this study was to simplify the AhpC protein purification procedures. Methods: For whole cell protein extraction, the bacterial cells were ruptured by octly-β-D glucopyranoside. The isolation and purification of  AhpC protein were attempted by various techniques including ammonium sulfate precipitation, dialysis, preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and electroelution. Results:A simple method was used for protein purification AhpC protein. One-dimensional preparative gel electrophoresis allows a single and short purification step; the high resolution capacity of this technique leads to a high level of purity of the protein. Moreover, it avoids contamination by other non-specific proteins which often appear during protein purification by column chromatography. Conclusion: The present method is simple, rapid and makes it possible to preparate AhpC from H. pylori https://ijph.tums.ac.ir/index.php/ijph/article/view/3138 https://ijph.tums.ac.ir/index.php/ijph/article/download/3138/2937