Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 39 4 2010 12 15 140 142 S Shatizadeh Malekshahi T Mokhtari Azad Sh Shahmahmoodi J Yavarian F Rezaei M Naseri 2015 10 03 Background: Respiratory virus infections in children are a leading cause of morbidity and mortality worldwide. Methods: A total of 897 clinical specimens were collected from February 2007 to January 2008 and transported to the Na­tional Influenza Center. Two hundred and two samples belonged to children under the age of six from 897 specimens, de­scribed above, were selected. Then they were tested for influenza virus types and subtypes by real time PCR assay subse­quently, the specimens were tested for RSV and hMPV by hemi-nested multiplex PCR and parainfluenza viruses type 1-4 by hemi-nested multiplex PCR and adenovirus by hemi-nested PCR. Results: The throat swab was taken from the Kawasaki case with the history of chicken`s contact. The specimen was tested for all influenza subtypes especially H5N1 and the results were negative. Meanwhile PCR was done for screening of other respira­tory viruses that results came out positive for RSV and hMPV. Conclusion: In the present study, we demonstrated the possibility to detect dual infection caused by RSV and hMPV, but be­cause of the extravagant pattern of this case, more investigation is suggested specially on Kawasaki patients. https://ijph.tums.ac.ir/index.php/ijph/article/view/3091 https://ijph.tums.ac.ir/index.php/ijph/article/download/3091/2890