Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 53 7 2024 07 07 1612 1620 Daniel Elieh-Ali-Komi 1. Institute of Allergology, Charité, Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Berlin, Germany 2. Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Allergology and Immunology, Berlin, Germany Tohid Kazemi 1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2. Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran Najibeh Shekari 1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2. Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran Parviz Farzamifar Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Elham Eghbali Medical Radiation Sciences Research Group, Tabriz University of Medical Sciences, Tabriz, Iran Behzad Mansoori 1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2. Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran Behzad Baradaran 1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2. Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran Masoud Shirmohamadi 1. Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2. Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran 2022 11 03 2023 07 31 Background: Linoleic acid (LA) has modulatory effects on gastric cancer cell lines. This study aimed to investigate the effects of linoleic acid on the expression of metastatic and angiogenic molecular markers in gastric cancer cell line MKN-45. Methods: In this study performed in Tabriz, Iran in 2021, MKN-45 cells were treated with LA in the presence or absence of docetaxel. Total RNA was extracted, and cDNA synthesized from the cells before and after treatment. The expression levels of Talin-2 and MMP-2 genes and mir-20, mir-30, mir-126, and mir-194, were determined by quantitative real-time PCR. Results: LA treatment reduced the expression levels of mir-126, mir-194, mir-30, and MMP-2, while increased the expression levels of Talin-2 mRNA. Docetaxel treatment could decrease the expression levels of mir-20, Talin-2, and MMP-2 mRNA levels while increasing the expression levels of mir-126, mir-194, and mir-30. Additionally, the combined treatment of MKN-45 cells with LA and docetaxel could reduce the expression levels of mir-20 and mir-126 and increased the expression levels of mir-194, mir-30, Talin-2, and MMP-2 mRNAs. Conclusion: Modulation of the expression levels of gastric cancer involved microRNAs, Talin-2, and MMP-2 may be a mechanism through which LA may exert its biological effects on GC cell line MKN-45. LA may have an antimetastatic effect by reducing the MMP-2 expression and pro-angiogenic effect through increasing Talin-2 expression levels. https://ijph.tums.ac.ir/index.php/ijph/article/view/30309 https://ijph.tums.ac.ir/index.php/ijph/article/download/30309/8293