Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 52 11 2023 10 29 2440 2449 Mehran Pourhossein Department of Occupational Health Engineering, School of Public Health, Zanjan University of Medical Sciences, Zanjan, Iran Monireh Khadem Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Fariborz Omidi Research Center for Environmental Determinants of Health, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran Omidreza Heravizadeh Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan Seyed Jamaledin Shahtaheri 1. Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2. Center for Water Quality Research, Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran 2022 10 01 2022 12 28 Background: A green sample preparation method named deep eutectic solvent-based single drop microextraction (DES-SDME) was developed and optimized for determining trace metribuzin, dichlorvos, and fenthion. Methods: Two hundred seventy experimental runs were performed, and the optimal values of the five influential factors in the DES-SDME method were determined. The design of the study was based on one factor at a time and the peak area of high-performance liquid chromatography was used as a benchmark for comparing analysis results. Results: After optimizing the effective factors, the linearity range, detection limit and quantification limit of the method were determined by drawing calibration curves for the studied analytes. Conclusion: The results indicated the success of the developed method in obtaining acceptable figures of merit as a green preparation method with accuracy and precision. https://ijph.tums.ac.ir/index.php/ijph/article/view/29974 https://ijph.tums.ac.ir/index.php/ijph/article/download/29974/8095