Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 12 1-4 1983 12 15 32 42 H.Mohammadiha P. Mostafavi 2015 10 03 A thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. The solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyridine-distilled water-glacial acetic acid. Procedures in all the three stages were in the same dimension. Results of casein analysis by this method showed ± 15% fluctuations when compared with standard amino acid analyzer. https://ijph.tums.ac.ir/index.php/ijph/article/view/2795 https://ijph.tums.ac.ir/index.php/ijph/article/download/2795/2775