Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 51 6 2022 06 08 1274 1282 Honghui Li Department of Occupational and Environmental Health, School of Public Health and Management, Ningxia Medical University, Yinchuan 750004, China Jiangping Li Department of Epidemiology and Health Statistic, School of Public Health and Management, Ningxia Medical University, Yin-chuan 750004, China Wei Sun Comprehensive Business Management Department of Ningxia Center for Disease Control and Prevention, Yinchuan 750004, China Lijun Dong Department of Occupational and Environmental Health, School of Public Health and Management, Ningxia Medical University, Yinchuan 750004, China Lingqin Zhu Department of Occupational and Environmental Health, School of Public Health and Management, Ningxia Medical University, Yinchuan 750004, China Zhen Jiang Yinchuan Guotai Hospital, Yinchuan 750001, Ningxia, China Huifang Yang Department of Occupational and Environmental Health, School of Public Health and Management, Ningxia Medical University, Yinchuan 750004, China 2021 11 08 2022 03 03 Background: To study the association between indoor environment (MiE), blood trace elements (BTE), and immune globulin (PRO) among workers from vegetables plastic greenhouse, and to assess the mediate effects on MiE and PRO by BTE. Methods: Overall, 168 practitioner and corresponding sheds were included from cross-sectional study in 2016. BTE and PRO were determined by physical test and MiE data from field and laboratory measurement. The association was assessed using canonical correlation analysis. The direct and indirect effects between MiE, PRO and BTE were conducted by structural equation model. 5000 times bootstrap methods were performed to estimate coefficient and 95% confidence interval. Results: MiE was moderately correlated with BTE (canonical coefficient = 0.439), and BTE was strongly correlated with PRO (canonical coefficient = 0.514 and 0.481). No statistical evidence was found for the overall impact of MiE on PRO, and BTE as an intermediary affecting its relevance was not confirmed. Only the path way of the BTE impact on PRO had a significant positive effect (P=0.012). Conclusion: BTE was positively associated with PRO, therefore, reducing exposure in greenhouse is a pathway to remain blood trace elements, and further effect the immune protein in human body.   https://ijph.tums.ac.ir/index.php/ijph/article/view/26806 https://ijph.tums.ac.ir/index.php/ijph/article/download/26806/7622