Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 41 8 2012 08 15 75 79 S Rezaei-Chaparpordi M Assmar N Amirmozafari L Modiri A Massiha S Shokri-Fashtali Z Gholizadeh S Akbari 2015 10 03 Background: Herpes simplex virus (HSV) type 1 and 2 are common infectious agents worldwide. Data on prevalence of HSV-1 and HSV-2 infection are limited in Asia, especially in Iran. The aim of this study was to determine the seroprevalence of HSV type 1 and 2 based on age, gender, marital status, education, living area, job, symptoms and history of disease variables. Methods: The study population included 800 randomly selected persons from laboratories in Gilan Province, Iran, from 2010 to 2011. Demographic data gathered by a well-designed questionnaire and for serological studies, blood samples were collected and centrifuged. ELISA HSV-1, 2 and HSV-2 specific ELISA kits were used to determine IgG type specific antibodies in sera samples. Person's chi-square test was applied to compare HSV-1 and HSV-2 seropositivities. Results: HSV-1 and HSV-2 IgG antibodies were positive in 467 (58.4%) and 28 (3.5%) subjects, respectively. There was significant correlation between age, marital status, job, symptoms, history of disease and HSV seroprevalence (P<0.05). Conclusion: Our findings were in agreement with prior studies in which HSV-1 infections was more prevalent than HSV-2 and seropositivity increased with age. https://ijph.tums.ac.ir/index.php/ijph/article/view/2535 https://ijph.tums.ac.ir/index.php/ijph/article/download/2535/2515