Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 51 7 2022 07 09 1538 1545 Mehdi Forozesh Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran Shiva Irani Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Azam Soleimani Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran Seyed Jalil Monabati Legal Medicine Research Center, Legal Medicine Organization, Tehran, Iran 2020 09 29 2021 06 21 Y-STR, DIP-STR, and SNP-STR are useful alternatives for testing the low quantity of DNA in solving the challenges in interpreting forensic genetic profiling. In an unbalanced mixed DNA, partial DNA is often not detected due to the effect of masking by the dominant DNA. Therefore, in such cases interpretation of the results is limited. Furthermore, profiling of these specimens cannot be performed using conventional forensic genetic methods. Biomarkers including Y-STR, DIP-STR and SNP-STR perform well in detecting DNA contributes in the mixed sample. In the present research, the performance of each is evaluated separately. https://ijph.tums.ac.ir/index.php/ijph/article/view/22369 https://ijph.tums.ac.ir/index.php/ijph/article/download/22369/7653