Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 35 1 2006 03 15 1 10 MR Aghasadeghi SM Sadat A Budkowska AR Khabiri S Amini G Bahramali SR Naddaf F Roohvand 2015 10 03 Infection with hepatitis C virus (HCV) is a worldwide problem. Among HCV proteins, core antigen (Ag), besides its importance for diagnostic application is a prime candidate for component of a vaccine. Herein, we report results of studies on production of the hydrophilic domain of core Ag (2-122) in native conformation by an arabinose induction system in E.coli and the primary characterization of this recombinant protein for applications in diagnosis, immunization and mAb production. Recombinant core (r-Core) was able to detect anti-core antibodies in HCV positive serum samples in a dilution rate of 1/3200. It was also capable to elicit a potent anti-HCV humoral immune response in BALB/c mice. Finally, we established two stable clones of hybridoma which shown to produce specific and sensitive mAbs against the core protein. HCV core was able to elicit a broad range of antibody specificities depending on the immunogen conformation. Therefore, it may be possible to get new mAbs with higher affinities towards native conformation of core Ag. https://ijph.tums.ac.ir/index.php/ijph/article/view/2184 https://ijph.tums.ac.ir/index.php/ijph/article/download/2184/2165