Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 37 3 2008 09 15 52 59 K Diba Dept. of Parasitology and Mycology, School of Public Health and Institute of Public Health Research SH Mirhendi Dept. of Parasitology and Mycology, School of Public Health and Institute of Public Health Research P Kordbacheh Dept. of Parasitology and Mycology, School of Public Health and Institute of Public Health Research N Jalalizand Laboratory of Molecular Biology, Esfehan Research Center of Public Health, Iran 2015 10 03 Background: Aspergillus species are associated with allergic bronchopulmonary disease, mycotic keratitis, otomycosis, na­sal sinusitis and invasive infection. In this study, we developed a PCR-Single Strand Confomational Polymorphism method to identify the most common Aspergillus species and we showed some advantages of this method comparing a PCR-Restric­tion Fragment Length Polymorphism with our designed restriction enzyme. Methods: We selected ITS2, as a short fragment within the rDNA region (length size: 330 bp) to be amplified as small size PCR product. We mixed 5 ml of the PCR product with an equal volume of loading buffer and followed by incubation for 5 min at 95º C and quenching in an ice bath. The mixture was applied to a 6%-12% Gradient Poly acryl amide gel to run in a verti­cal electrophoresis, then gel was stained with ethidium bromide and silver nitrate which followed by an ethidium bro­mide staining. Results: Our results of restriction digestion showed a fine identification of 7 tested Aspergillus species dur­ing 5-6 hours af­ter an overnight mycelial growth. As our results some of tested Aspergillus species: A. nidulans, A. fisheri, A. quad­ricincta, (A. fumigatus and A. niger) as a group and (A. flavus, A. tereus and A. ochraceus) as another group, can be dis­criminated. More­over SSCP analysis enabled us to identify above Aspergillus species within 8-12 h after an over night growth without us­ing an expensive restriction enzyme. Conclusion: It is concluded that Single Strand Conformational Polymorphism is a simple and rapid method for identifica­tion of some medically important Aspergillus. https://ijph.tums.ac.ir/index.php/ijph/article/view/2034 https://ijph.tums.ac.ir/index.php/ijph/article/download/2034/2015