Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 37 4 2008 12 15 96 102 B Kazemi Cellular and Molecular Biology Research Center, Shahid Beheshti University, M. C., Tehran, Iran SA Yousefi Namin Islamic Azad University, Jahrom, Iran M Bandepour Cellular and Molecular Biology Research Center, Shahid Beheshti University, M. C., Tehran, Iran F Kafilzadeh Islamic Azad University, Jahrom, Iran L Gachkar Infectious Disease and Tropical Medicine Research Center, Shahid Beheshti University, M.C., Tehran, F Mahmoudinejad Islamic Azad University, Jahrom, Iran A Samarghandi Cellular and Molecular Biology Research Center, Shahid Beheshti University, M. C., Tehran, Iran M Mardani Infectious Disease and Tropical Medicine Research Center, Shahid Beheshti University, M.C., Tehran, 2015 10 03 Background: Brucellosis is a zoonotic disease and its symptoms and appearances are not exclusive in human and its tradi­tional diagnosis is based on blood culture and serological methods. For more sensitive and specific detection, the PCR method is recommended. Methods: One hundred four blood samples were gathered from suspicious patients with brucellosis different ages form Ker­manshah, Mazandaran, Khorassan and Hormozgan provinces, and were examined by Rose Bengal, anti globulin, culture and PCR methods. Results: Seventy three samples were positive by PCR method, 15 samples were positive by cultured method and 84 sam­ples were positive by serological methods. Conclusion:  PCR method is sensitive and specific for diagnosis of Brucella from peripheral blood in suspected cases.   https://ijph.tums.ac.ir/index.php/ijph/article/view/2020 https://ijph.tums.ac.ir/index.php/ijph/article/download/2020/2001