Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 50 8 2021 07 22 1697 1704 EN Fatemeh Esfahanian Department of Endocrinology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran Mojgan Mirabdolhagh Hazaveh Department of Internal Medicine, Sina Hospital, Tehran University of Medical Sciences, Tehran, Iran Laya Hooshmand Garehbagh Department of Endocrinology, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran Kowsar Falahati Clinical Genetics Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Mitra Ataei Clinical Genetics Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Mohammad Hossein Sanati Clinical Genetics Department, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran Zohreh Jadali Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2020 01 19 2020 05 05 Background: The purpose of present study was to investigate mitochondrial DNA copy number (mtDNAcn) and mtDNA damage in peripheral blood of patients with Hashimoto's thyroiditis (HT) and healthy controls (HC). Methods: The relative mtDNAcn and oxidative DNA damage in this case-control study were measured in peripheral blood of 50 patients with Hashimoto’s thyroiditis and 50 healthy controls using quantitative real-time PCR. The study was conducted in Tehran University of Medical Sciences hospital, Tehran, Iran in 2018. Results: HT patients had significantly higher mitochondrial DNA copy number and mitochondrial oxidative damage than the comparison group. Conclusion: These data suggest the possible involvement of mitochondria and oxidative stress in the pathophysiology of HT. https://ijph.tums.ac.ir/index.php/ijph/article/view/19484 https://ijph.tums.ac.ir/index.php/ijph/article/download/19484/7328