Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 49 1 2020 01 07 173 180 Neveen Magdy RIZK Environmental Virology Lab., Department of Water Pollution Research, National Research Center, Giza, Egypt Abdou Kamal ALLAYEH Environmental Virology Lab., Department of Water Pollution Research, National Research Center, Giza, Egypt 2020 01 07 Background: In 2013, WHO estimated the annual diarrheal mortality rate among children less than five years in Egypt was 24 deaths per 100.000, 2% was due to rotavirus infection. Methods: Eight water samples were collected monthly from the Nile water stream passing through Giza over 12 months during June 2016 to May 2017. Totally, ninety-six water samples were collected and concentrated for the detection of rotavirus group A (RV-A) using RT-PCR. Multiplex semi-nested RT-PCR was performed to identify the genotypes P and G of RV-A. Results: The detection rate of RV-A was 18.75% (18/96), whereas the rate of rotavirus genotypes G and P were 61% (11/18) and 50% (9/18), respectively. Rotaviruses G1P[8] and G1P[4] were the most common genotypes identified in our survey. In addition, the seasonal distribution findings demonstrated that the highest detection rate was 37.5% in the winter season, followed by 20.8%, 12.5% and 4% in spring, autumn and summer, respectively. Conclusion: Multiplex semi-nested RT-PCR is a useful method for rapid detection and genotyping of RV-A in surface water samples. https://ijph.tums.ac.ir/index.php/ijph/article/view/19376 https://ijph.tums.ac.ir/index.php/ijph/article/download/19376/6655