Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 49 1 2020 01 07 125 133 Amir JAVADI Department of Health Information Management, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran AND Department of Medical Social Sciences, Faculty of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran Ali KHAMESIPOUR Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran Farshid MONAJEMI Ministry of Health and Medical Education, Tehran, Iran Marjan GHAZISAEEDI Department of Health Information Management, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran 2020 01 07 Background: In a new approach, computational methods are used to design and evaluate the vaccine. The aim of the current study was to develop a computational tool to predict epitope candidate vaccines to be tested in experimental models. Methods: This study was conducted in the School of Allied Medical Sciences, and Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, Iran in 2018. The random forest which is a classifier method was used to design computer-based tool to predict immunogenic peptides. Data was used to check the collected information from the IEDB, UniProt, and AAindex database. Overall, 1,264 collected data were used and divided into three parts; 70% of the data was used to train, 15% to validate and 15% to test the model. Five-fold cross-validation was used to find optimal hyper parameters of the model. Common performance metrics were used to evaluate the developed model. Results: Twenty seven features were identified as more important using RF predictor model and were used to predict the class of peptides. The RF model improves the performance of predictor model in comparison with the other predictor models (AUC±SE: 0.925±0.029). Using the developed RF model helps to identify the most likely epitopes for further experimental studies. Conclusion: The current developed random forest model is able to more accurately predict the immunogenic peptides of intracellular parasites. https://ijph.tums.ac.ir/index.php/ijph/article/view/19369 https://ijph.tums.ac.ir/index.php/ijph/article/download/19369/6637