Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 32 4 2003 12 15 8 12 AA Eskandarian H Keshavarz F Mahboudi 2015 10 03 Using mutation specific PCR assay and analysis of position 108 amino acid in dihydrofolate reductase-thymidilate synthase gene in 70 Plasmodium falciparum isolates of malarial patients from southern areas of Iran, showed 4 different situations: iwild type (108-Ser) ii-mutant type (108-Thr) iii-mutant type (108-Asn) and iv-mixed wild and mutant type (108-Ser+Thr) with frequency of 58(82.8%), 2(2.9%), 8(11.4%) and 2(2.9%) respectively. Many studies have demonstrated that mutations in position 108 have key role for emerging resistance to anti-folate drugs in falciparum malaria so, the resistance to pyrimethamine and proguanil, presents in Plasmodium falciparum isolates from malarious endemic regions of Iran. https://ijph.tums.ac.ir/index.php/ijph/article/view/1933 https://ijph.tums.ac.ir/index.php/ijph/article/download/1933/1914