Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 49 7 2020 06 30 1298 1306 Liqin SHAO Department of Ophthalmology, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang 312000, China Zhangxing SHENG Department of Ophthalmology, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang 312000, China Yuefeng ZHU Department of Ophthalmology, Affiliated Hospital of Shaoxing University, Shaoxing, Zhejiang 312000, China Jianchao LI Department of Ophthalmology, Xi'an Traditional Chinese Medicine Hospital, Xi'an, Shaanxi 710020, China Rufa MENG Department of Ophthalmology, The Fifth Hospital of Shaoxing, Shaoxing, Zhejiang 312000, China 2019 11 29 2020 06 16 Background: Effect of miR-215 on the expression of tumor suppressor gene retinoblastoma (Rb)1 in Rb cell lines was investigated. Methods: A total of 128 patients were selected. The expression of miR‑215 in cancer and adjacent healthy tissues of the 128 patients was detected by reverse transcription-quantitative PCR (RT-qPCR). HXO‑Rb44 and Y79 cell lines were transfected with miR‑215 analogs or miR‑215 inhibitors, and the expression of Rb1 protein in the cell lines was detected by western blotting. Results: The expression of miR-215 in the adjacent healthy tissues of patients was significantly lower than that in cancer tissues (P<0.001). The expression of miR-215 in Y79 and HXO-Rb44 cells was significantly higher than that in APRE-19 cells (P<0.001). The expression of miR-215 in HXO-Rb44 cells was significantly higher than that in Y79 cells (P<0.001). The expression of miR-215 was statistically different from the degree of differentiation and nerve infiltration (P<0.05). The expression of Rb1 in cancer tissues was significantly lower than that in adjacent tissues (P<0.001), the expression of APRE-19 was significantly higher than that in Y79 and HXO-Rb44 cells (P<0.001), and the expression of Rb1 in HXO-Rb44 cells was significantly higher than that in Y79 cells (P<0.05). There was a negative correlation between miR-215 and Rb1 in the tissues of patients, and Rb1 expression decreased with the increase of miR-215 (r=-0.576, P<0.001). Conclusion: miR‑215 is highly expressed in Rb cell lines, and is related to the clinicopathological features of this disease. https://ijph.tums.ac.ir/index.php/ijph/article/view/19024 https://ijph.tums.ac.ir/index.php/ijph/article/download/19024/6855