Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 34 3 2005 09 15 27 30 N Zia-Ali H Keshavarz-Valian M Rezaian MR Khorramizadeh B Kazemi A Fazaeli M Darde 2015 10 03 The determination of Toxoplasma gondii prevalence in a variety of domestic birds is thought as a good indicator of distribution of T.gondii ooccyts in the environment. To verify the potential role of domestic birds in circulating toxoplasmosis in Iran, the present study was conducted in Mazandaran Provinces, northern Iran. Latex agglutination test (LA) antibodies were found in 25 of 58 free-ranging chickens (Gallus domesticus) and ducks (Anas sp.). Also, viable T. gondii was isolated from 7 of 25 seropositive chickens and ducks by bioassay of their brains and hearts into mice. Most of the isolated strains were avirulant to mice. Genotyping of T.gondii isolates using Multiplex PCR for 5 microsatellite markers indicated that 7 isolates were type III. In this study type II and III isolates and mixed genotypes were not found. This study showed that domestic birds could have a potential role in transmitting toxoplasmosis to humans in Iran. https://ijph.tums.ac.ir/index.php/ijph/article/view/1851 https://ijph.tums.ac.ir/index.php/ijph/article/download/1851/1832