Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 48 3 2019 03 12 501 507 Ahmad HOSSEINI-SAFA Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mohammad Bagher ROKNI Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Sayed Hussain MOSAWI Department of Biology and Microbiology, Faculty of Medical Technology, Khatam Al-Nabieen University, Kabul, Afghanistan Peyman HEYDARIAN Department of Medical Parasitology and Mycology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran Hakim AZIZI Department of Microbiology and Parasitology, School of Medicine, Zabol University of Medical Sciences, Zabol, Iran Afshin DAVARI Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Mojgan ARYAIEPOUR Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran AND Department of Medical Parasitology and Mycology, Shahid Beheshti University of Medical Sciences, Tehran, Iran 2019 03 12 Background: Fasciolosis is a shared disease between humans and livestock caused by hepatic trematodes; Fasciola hepatica and F. gigantica. Differentiate between the two species of this genus is essential. High-Resolution Melting (HRM) Analysis represents a new approach to this issue. This method can be performed right after termination of Real-Time PCR. This technique has not been used for identification of adult F. hepatica and F. gigantica genotypes. The aim of this study was to determine Fasciola species by using HRM in isolates taken from Iran, respectively. Methods: Ninety-three Fasciola spp. samples were collected from infected slaughtered animals in different regions of Iran, including North West (Ardebil Province) and South East (Zahedan Province) during 2016. Genomic DNA from the samples was extracted using a DNA extraction kit and then after Real-Time PCR amplification, HRM was done. Results: Overall, 59 and 34 isolates were identified as F. hepatica and F. gigantica, respectively. The percentages of each species from animals were as follows: sheep (F. hepatica, 80.39% and F. gigantica, 19.61%), cattle (F. hepatica, 42.85% and F. gigantica, 57.15%). Conclusion: HRM technique developed in the present study is a powerful, rapid and sensitive technique for epidemiological survey and molecular identification between F. hepatica and F. gigantica.   https://ijph.tums.ac.ir/index.php/ijph/article/view/16533 https://ijph.tums.ac.ir/index.php/ijph/article/download/16533/6329