Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 49 8 2020 07 30 1546 1554 EN Roya KOLAHDOUZ-MOHAMMADI Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran Ali-Akbar DELBANDI Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran AND Immunology Research Center, Institute of Immunology and Infectious Diseases, Iran University of Medical Sci-ences, Tehran, Iran Sepideh KHODAVERDI Endometriosis Research Center, Iran University of Medical Sciences, Tehran, Iran Soheila AREFI Bahman Hospital Infertility Center, Tehran, Iran AND Genetics and In Vitro Assisted Reproductive (GIVAR) Center, Erfan Hospital, Tehran, Iran Tahereh ARABLOU Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran Farzad SHIDFAR Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran 2018 10 20 2018 12 02 Background: We aimed to examine resveratrol effects on gene expression of Bcl-2, Bax and Bcl-2/Bax ratio in endometrial stromal cells derived from women with and without endometriosis. Methods: Endometrial tissues were obtained from 40 endometriotic patients and 15 non-endometriotic controls undergoing laparoscopic surgery or hysterectomy in the gynecology ward of Rassoul Akram Hospital, Tehran, Iran from 2015 to 2017. After the enzymatic digestion, eutopic (EuESCs) and ectopic (EESCs) endometrial stromal cells from patients with endometriosis as well as endometrial stromal cells from non-endometriotic controls (CESCs) were treated with or without resveratrol (100 µM) and the levels of Bcl-2, Bax and Bcl-2/Bax gene expression ratio in the cells from all origins were examined at 6, 24 and 48 h post-treatment by real-time PCR. Results: Resveratrol treatment increased Bcl-2 expression in CESCs at 24 and 48 h and in EuESCs at 48 h (P<0.05), but had no significant effects on the expression of this gene in EESCs. On the other hand, resveratrol treatment increased Bax expression in EuESCs at 6 h and decreased its expression in EESCs at 48 h (P<0.05). Regarding the Bcl-2/Bax gene expression ratio, resveratrol treatment increased Bcl-2/Bax gene expression ratio in CESCs and EuESCs at 48 h (P<0.01). However, this treatment had no significant differential effect on Bcl-2 and Bcl-2/Bax gene expression ratio between CESCs and EuESCs at 48 h. Conclusion: Resveratrol treatment significantly increased Bcl-2/Bax gene expression ratio in EuESCs and CESCs but had no significant effect in EESCs. https://ijph.tums.ac.ir/index.php/ijph/article/view/15052 https://ijph.tums.ac.ir/index.php/ijph/article/download/15052/6949