Tehran University of Medical Sciences Iranian Journal of Public Health 2251-6085 46 12 2017 11 29 1632 1638 Jianguo SUN People’s Hospital of Rizhaolanshan, Rizhao, Shandong, PR China Hui ZHENG People’s Hospital of Wulian, Rizhao, Shandong, PR China Xiuxia QIN People’s Hospital of Rizhaolanshan, Rizhao, Shandong, PR China Liqin QI People’s Hospital of Rizhaolanshan, Rizhao, Shandong, PR China 2017 11 29 2017 11 29  Background: This study was designed to explore the correlation of connective tissue growth factor (CTGF), heme oxygenase (HO-1), neurotrophic factors (NT-3) with type 2 diabetic peripheral neuropathy, as well as the changes after immune cytokine alone and combined with cattleencephalon glycoside and ignotin treatment. Methods: Seventy-six patients with type 2 diabetes and peripheral neuropathy charged into People’s Hospital of Rizhaolanshan, China from 2014-2016 were selected. The severity of neuropathy was evaluated by TCSS. Pearson analysis was used to analyze the correlation between the degree of neuropathy and CTGF, HO-1 and NT-3. The patients were randomly divided into control group and observation group, n=38. The control group accepted TGF-β1 treatment on the basis of controlling diet and blood sugar, while the observation group was treated with cattle encephalon glycoside and ignotin injection on the basis of control group. CTGF、HO-1, NT-3 concentration in the blood and nerve conductive velocity (NCV) were detected and analyzed before and after treatment. Results: CTGF(r=-0.865), HO-1(r=-0.706), NT-3(r=-0.587) was negatively correlated with TCSS scores. After treatment, the concentrations of CTGF、HO-1and NT-3 in the observation group were higher than the control group (P<0.05). In moderate and severe lesions, the concentrations of CTGF, HO-1and NT-3 in the observation group were higher than the control group (P<0.05). The conduction velocity of nerve increased with the increase of CTGF, HO-1 and NT-3 concentrations. The obvious effective rate and total effective rate of observation group were both higher than the control group. Conclusion:  Immune cytokine TGF-β1 combined with cattle encephalon glycoside and ignotin injection could improve the contents of CTGF, HO-1 and NT-3, and be better to treat the peripheral neuropathy of type 2 diabetes.     https://ijph.tums.ac.ir/index.php/ijph/article/view/11758 https://ijph.tums.ac.ir/index.php/ijph/article/download/11758/5842