Iranian Journal of Public Health 1983. 12(1-4):32-42.

ESTIMATION OF METHIONINE AND LYSINE IN PROTEIN BY THIN-LAYER CHROMATOGRAPHY
H.Mohammadiha, P. Mostafavi

Abstract


A thin-layer chromatography method has been developed and evaluated for the separation and determination of lysine and methonine in a mixture of amino acids obtained from acid or enzyme hydrolysis of protein. The solvent systems were (a) ethanol-ether, (b) phenol-water, (c) ethyl methyl ketone-pyridine-distilled water-glacial acetic acid. Procedures in all the three stages were in the same dimension. Results of casein analysis by this method showed ± 15% fluctuations when compared with standard amino acid analyzer.

Keywords


Methionine, Lysine, Thin-layer chromatography,

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