Identification of Nonylphenol and Glucolipid Metabolism-Related Proteins in the Serum of Type 2 Diabetes Patients
Background: To identify serum nonylphenol (NP) and glucolipid metabolism-related proteins in Type 2 diabetes (T2D) patients.
Methods: We performed a hospital-based, case-control study in patients admitted to the Department of Endocrinology, Hospital of Zunyi Medical University, Zunyi City, China from Mar to Nov of 2014. The study included 112 T2D cases diagnosed in accordance with the 2013 WHO Expert Committee Diabetes Diagnosing Criteria, and 125 healthy individuals with normal fasting blood glucose (FBG) when receiving physical examination in the same period in the Municipal Physical Examination Center. Blood samples from subjects in the 2 groups underwent detection of biochemical indices, including FBG, blood fat, and NP. Glucolipid metabolism-related proteins, including estrogen receptor (ER), sterol regulatory element-binding protein-1c (SREBP-1c), wingless-type MMTV integration site family member 5a (Wnt5a), and peroxisome proliferator-activated receptor-γ (PPAR-γ). These indices were compared between the 2 groups to analyze the correlation between serum NP levels and glucolipid metabolic proteins.
Results: The subjects in the diabetes group had higher triglycerides (TG), total cholesterol (TC), NP, ER, SREBP-1c, Wnt5a, FBG, and TG levels than the healthy group, but lower levels of low-density lipoprotein cholesterol (LDL-C) and PPAR-γ than the healthy group. No significant differences in alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were found between the two groups. The serum NP levels were shown to be positively correlated with SREBP-1c but negatively correlated with PPAR-γ.
Conclusion: The serum NP levels of T2D patients is higher than the levels in healthy controls, and its levels correlate with SREBP-1c and PPAR-γ levels.
2. Sophie Cousins (2017). Address-ing Asia’s fast growing diabetes epidemic. Bull World Health Organ, 95(8):550-51.
3. Yang J, Yu J, Yang MX, et al (2016). Rela-tionship between environmental estrogen exposure and diabetes mellitus: a review. Chin J Public Health, 32(07):988-90.
4. Huang YF, Wang PW, Huang LW, et al (2014). Nonylphenol in pregnant women and their matching fetuses: Placental transfer and potential risks of infants. Environ Res, 134:143-48.
5. Wang PW, Chen ML, Huang LW, et al (2015). Nonylphenol exposure is associ-ated with oxidative and nitrative stress in pregnant women. Free Radic Res, 49(12):1469-78.
6. Luo Y, Zheng GB, Yang XF, et al (2016). The load of serum nonylphenol in pa-tients with type-2 diabetes mellitus. J Pract Med, 32(13):2227-30.
7. Li WM, YU J, Luo Y, Xu J, et al (2017). Ef-fect of NP exposure on neonatal rat obe-sity of pregnant rats induced by high-fat and high-glucose diet during perinatal pe-riod. Shandong Medical Journal, 57(14):18-22.
8. Jie Y, Xuefeng Y, Mengxue Y, et al (2016). Mechanism of nonylphenol-induced neu-rotoxicity in F1 rats during sexual maturi-ty. Wien Klin Wochenschr, 128(11-12):426-34.
9. Jie X, Jianmei L, Zheng F et al (2013). Neu-rotoxic effects of nonylphenol: a review. Wien Klin Wochenschr, 125(3-4):61-70.
10. Shahsavarian A, Javadi S, Jahanabadi S, et al (2014). Antidepressant-like effect of atorvastatin in the forced swimming test in mice: The role of PPAR-gamma recep-tor and nitric oxide pathway. Eur J Phar-macol, 745:52-8.
11. Wu SJ, Gao LP (2014). Effect of diet control combined with aerobic exercise on TNF-α,SREBP-1c levels in patients with non-alcoholic fatty liver. China Modern Doctor, 52(33):4-8
12. Tong X, Li P, Zhang D, et al (2016). E4BP4 is an insulin-induced stabilizer of nuclear SREBP-1c and promotes SREBP-1c-mediated lipogenesis. J Lipid Res, 57(7):1219-30.
13. Kimura Y, Tamasawa N, Matsumura K, et al (2016). Clinical Significance of Determin-ing Plasma MicroRNA33b in Type 2 Di-abetic Patients with Dyslipidemia. J Ather-oscler Thromb, 23(11):1276-85.
14. Ge JF, Walewski JL, Anglade D, et al (2016). Regulation of Hepatocellular Fatty Acid Uptake in Mouse Models of Fatty Liver Disease with and without Functional Leptin Signaling: Roles of NfKB and SREBP-1C and the Effects of Spexin. Semin Liver Dis, 36 (4):360-72.
15. Zhang HY，Gao YX，Feng XF, et al (2014). Research on correlation between gene polymorphism of sterol regulatory element binding protein 1c and non-alcoholic fatty liver disease. Xinan Guofang Yiyao, 24(2):117-20.
16. PU P (2016). Protection mechanisms of hesperidin on mouse with insulin re-sistance. Zhongguo Zhong Yao Za Zhi, 41(17):3290-95.
17. PaneniF, Costantino S, Cosentino F (2014). Insulin resistance, diabetes, and cardio-vascular risk. Curr Atheroscler Rep, 16(7):419.
18. Al-Goblan AS, Al-Alfi MA, Khan MZ (2014). Mechanism linking diabetes melli-tus and obesity. Diabetes Metab Syndr Obes, 7: 587–91.
19. Kang B, Zuo aGP, He S (2016). Relationship between serum Wnt5a and fasting plasma glucose inpatients with type 2 deabetes. Chin J Diabetes, 24(8): 686-88.
20. Farb MG, Karki S, Park SY, et al (2016). WNT5A-JNK regulation of vascular in-sulin resistance in human obesity. Vasc Med, 21(6):489-96.