Development of Dispersive Liquid-Liquid Microextraction Procedure for Trace Determination of Malathion Pesticide in Urine Samples

  • Maryam RAMIN Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Monireh KHADEM Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Fariborz OMIDI Research Center for Environmental Determinants of Health (RCEDH), Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran
  • Mehran POURHOSEIN Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Farideh GOLBABAEI Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Seyed Jamaleddin SHAHTAHERI 1. Department of Occupational Health Engineering, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran 2. Institute for Environmental Research, Tehran University of Medical Sciences, Tehran, Iran
Keywords: Malathion; Dispersive liquid-liquid microextraction; One variable at a time; Urine; HPLC

Abstract

Abstract Background: Measurement of pesticides in biological matrices is become a serious challenge for researches because of their very low concentration in different matrices. The aim of this study was to develop a new sample preparation method with high accuracy and validity, simplicity and short retention time for determination of malathion. Methods: Dispersive liquid-liquid micro-extraction (DLLME) technique coupled with high-performance liquid chromatography equipped with ultraviolet detector (HPLC-UV) developed for trace extraction and determination of malathion pesticide in human urine samples. This study was done in 2017 at Tehran University of Medical Sciences, Tehran, Iran. One variable at a time (OVAT) method was used to optimize parameters affecting the malathion extraction. Different parameters such as extraction solvent, disperser solvent, and volume of the extraction solvent, volume of the disperser solvent, centrifugation time and speed, salt addition, and sample pH were studied and optimized. Results: Under the optimized conditions, the limit of detection and enrichment factor of the method were 0.5 µg L-1 and 200, respectively. The calibration curve was linear in the concentration range of 2-250 µg L-1. The relative standard deviation for six replicate experiments at 200 µg L-1 concentration was less than 3%. The relative recoveries of spiked urine samples were 96.3%, 101.7% and 97.3% at three different concentration levels of 50, 200 and 1000 µg L-1, respectively. Conclusion: DLLME procedure was successfully developed for the extraction of malathion from human urine samples. Compared to other extraction techniques, the proposed procedure had some advantages such as shorter extraction time, better reproducibility, and higher enrichment factor.    

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Published
2019-10-05
How to Cite
1.
RAMIN M, KHADEM M, OMIDI F, POURHOSEIN M, GOLBABAEI F, SHAHTAHERI SJ. Development of Dispersive Liquid-Liquid Microextraction Procedure for Trace Determination of Malathion Pesticide in Urine Samples. Iran J Public Health. 48(10):1893-1902.
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Original Article(s)