Mechanism of Probiotic VSL#3 Inhibiting NF-κB and TNF-α on Colitis through TLR4-NF-κB Signal Pathway

  • Hui WANG Department of Immunology, Qiqihar Medical University, Qiqihar 161000, P.R. China
  • Shuhua LI Department of Traditional Chinese Medicine, The First Hospital of Qiqihar, Qiqihar 161000, P.R. China
  • Houzhong LI Department of Pharmacology, Mudanjiang Medical University, Mudanjiang 157011, P.R. China
  • Fengxia DU Department of Pathogen Biology, Qiqihar Medical University, Qiqihar 161000, P.R. China
  • Jie GUAN Department of Immunology, Qiqihar Medical University, Qiqihar 161000, P.R. China
  • Yanmin WU Department of Immunology, Qiqihar Medical University, Qiqihar 161000, P.R. China
Keywords: Probiotics VSL#3; NF-κB; TLR4; TNF-α

Abstract

Abstract Background: We aimed to investigate the effect of probiotic VSL#3 on NF-κB and TNF-α in rats with colitis and the correlation with TLR4-NF-κB signal pathway. Methods: Sixty Sprague Dawley (SD) rats were divided into the control, model and therapy groups (n=20) according to the random number table. Rats in the model and therapy groups were modeled for colitis, and rats in the therapy group were intragastrically administered with probiotic VSL#3. The expression of TLR4 and NF-κB protein, and the levels of NF-κB, TLR4, and TNF-α mRNA in the colon tissue were detected. The concentration of TNF-α in the serum after modeling but before intragastric administration (T0), 3d (T1) and 7d after intragastric administration (T2) was detected. Results: The expression of TLR4 and NF-κB p65 protein, and the levels of TLR4, NF-κB, and TNF-α mRAN in the therapy group decreased (P < 0.001). At T0, T1, and T2, the concentration of TNF-α in the model and control groups increased (P < 0.001). TLR4 and NF-κB in the therapy group were positively correlated with TNF-α mRAN (P < 0.050).Conclusion: In conclusion, probiotic VSL#3 inhibits the expression of NF-κB and TNF-α in rats with colitis through TLR4-NF-κB signal pathway, so it is expected to be a first choice drug for the treatment of colitis. Conclusion: In conclusion, probiotic VSL#3 inhibits the expression of NF-κB and TNF-α in rats with colitis through TLR4-NF-κB signal pathway, so it is expected to be a first choice drug for the treatment of colitis.  

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Published
2019-07-01
How to Cite
1.
WANG H, LI S, LI H, DU F, GUAN J, WU Y. Mechanism of Probiotic VSL#3 Inhibiting NF-κB and TNF-α on Colitis through TLR4-NF-κB Signal Pathway. Iran J Public Health. 48(7):1292-1300.
Section
Original Article(s)