The Association between SIRT1 Genetic Variation and Type 2 Diabetes Mellitus Is Influenced by Dietary Intake in Elderly Chi-nese

  • Zeng ZHUANPING School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China
  • Liao RIFANG Dept. of Pharmacy, Sun Yat-Sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China
  • Chen QING Dept. of Epidemiology, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou 510515, China
  • Chen SIDONG School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510310, China
Keywords: Sirtuin 1, Type 2 diabetes mellitus, Dietary, China


AbstractBackground: To examine whether polymorphisms of SIRT1 and dietary product intake can be implicated in type 2 diabetes mellitus (T2DM).Methods: In this community-based, case-control study, 568 subjects (284 patients and 284 controls) were enrolled in a community located in northern Guangzhou, China. The four polymorphisms of SIRT1 (rs4746720, rs10509291, rs2236319, rs10823116) were examined using TaqMan nuclease technology. The dietary data were collected by an inquiring officer through face-to-face method.Results: The rs4746720 CC+TT genotype had higher risk compared with CT genotype to develop T2DM [odds ratio (OR) =1.42, 95% confidence interval (CI) = 1.02-1.97]. The subjects with rs4746720 CC+TT genotype and eat sugar food over 30g per day increased the risk of T2DM to 2.22(1.21-4.06) times. The subjects with rs4746720 CC+TT genotype and smoking increased the risk of T2DM to 1.65 (1.10-2.47) times. The unhealthy eating habits such as red meat, salty food, use animal fat yielded higher risks of T2DM, the OR of risk of T2DM was 2.89 (1.38-6.01), 2.73 (1.61-4.64) and 27.91(9.24-84.32) respectively. However, the milk, soy, white meat, vegetables and low-salt diet decreased the risk of T2DM, the OR of risk of T2DM was 0.51 (0.29-0.88), 0.43 (0.26-0.74), 0.51(0.32-0.83), 0.21(0.10-0.44), 0.28(0.12-0.65), 0.35(0.21-0.51) respectively.Conclusion: Variants in SIRT1 with rs4746720 CC+TT genotype increased the risk of T2DM, especially with the unhealthy eating habits. 


Meng J, Casciano R, Lee YC et al (2017). Effect of Diabetes Treatment-Related Attributes on Costs to Type 2 Diabetes Patients in a Real-World Population. J Manag Care Spec Pharm, 23:446-452.

Yang L, Shao J, Bian Y et al (2016). Prevalence of type 2 diabetes mellitus among inland residents in China (2000-2014): A meta-analysis. J Diabetes Investig, 7:845-852.

Chiefari E, Tanyolac S, Paonessa F et al (2011). Functional variants of the HMGA1 gene and type 2 diabetes mellitus. JAMA, 305:903-12.

Chon SJ, Kim SY, Cho NR et al (2013). Association of variants in PPARgamma(2), IGF2BP2, and KCNQ1 with a susceptibility to gestational diabetes mellitus in a Korean population. Yonsei Med J, 54:352-7.

Kaul N, Singh YP, Bhanwer AJ (2015). The influence of ethnicity in the association of WC, WHR, hypertension and PGC-1alpha (Gly482Ser), UCP2 -866 G/A and SIRT1 -1400 T/C polymorphisms with T2D in the population of Punjab. Gene, 563:150-4.

Li H, Gan W, Lu L et al (2013). A genome-wide association study identifies GRK5 and RASGRP1 as type 2 diabetes loci in Chinese Hans. Diabetes, 62:291-8.

Blander G, Olejnik J, Krzymanska-Olejnik E et al (2005). SIRT1 shows no substrate specificity in vitro. J Biol Chem, 280:9780-9785.

Kume S, Uzu T, Kashiwagi A, Koya D (2010). SIRT1, a calorie restriction mimetic, in a new therapeutic approach for type 2 diabetes mellitus and diabetic vascular complications. Endocr Metab Immune Disord Drug Targets, 10:16-24.

Kitada M, Koya D (2013). SIRT1 in Type 2 Diabetes: Mechanisms and Therapeutic Potential. Diabetes Metab J, 37:315-25.

Ishikawa S, Li G, Takemitsu H et al (2013). Change in mRNA expression of sirtuin 1 and sirtuin 3 in cats fed on high fat diet. BMC Vet Res, 9:187.

Liu M, Li L, Chu J et al (2016). OS 33-03 serum n1-methylnicotinamide is associated with obesity and diabetes in chinese. J Hypertens, 34(e-Suppl 1):e393-e394.

Lovegrove JA, Givens DI (2016). Dairy food products: good or bad for cardiometabolic disease? Nutr Res Rev, 29(2):249-267.

Musa-Veloso K, Paulionis L, Poon T, Lee HY (2016). The effects of almond consumption on fasting blood lipid levels: a systematic review and meta-analysis of randomised controlled trials. J Nutr Sci, 5:e34.

Raabo E, Terkildsen TC (1960). On the enzymatic determination of blood glucose. Scand J Clin Lab Invest, 12:402-7.

Davidson M, Liu SX, Barter P et al (2013). Measurement of LDL-C after treatment with the CETP inhibitor anacetrapib. J Lipid Res, 54:467-72.

WHO (2000). Obesity: preventing and managing the global epidemic. Report of a WHO consultation.

Zheng J, Chen L, Xiao F et al (2012). Three single nucleotide variants of the SIRT1 gene are associated with overweight in a Chinese population: a case control study. Endocr J, 59:229-237.

Price NL, Gomes AP, Ling AJY et al (2012). SIRT1 is required for AMPK activation and the beneficial effects of resveratrol on mitochondrial function. Cell Metab, 15: 675–690.

Zhang W-G, Bai X-J, Chen X-M (2010) SIRT1 variants are associated with aging in a healthy Han Chinese population. Clin Chim Acta, 411(21-22):1679-83.

Zillikens MC, van Meurs JBJ, Sijbrands EJG et al (2009). SIRT1 genetic variation and mortality in type 2 diabetes: interaction with smoking and dietary niacin. Free Radic Biol Med,46(6):836-41.

Dong Y, Guo T, Traurig M et al (2011). SIRT1 is associated with a decrease in acute insulin secretion and a sex specific increase in risk for type 2 diabetes in Pima Indians. Mol Genet Metab,104(4):661-5.

Montonen J KP, Härkänen T, Järvinen R et al (2005). Dietary patterns and the incidence of type 2 diabetes. Am J Epidemiol,161(3):219-27.

Villegas R, Yang G, Gao Y-T et al (2010). Dietary patterns are associated with lower incidence of type 2 diabetes in middle-aged women: the Shanghai Women's Health Study. Int J Epidemiol,39(3): 889–899.

Lopez-Ridaura R, Willett WC, Rimm EB, Liu S, Stampfer MJ, Manson JE, Hu FB (2004). Magnesium intake and risk of type 2 diabetes in men and women. Diabetes Care, 27(1):134-40.

Schulze MB, Schulz M, Heidemann C, Schienkiewitz A, Hoffmann K, Boeing H (2007). Fiber and magnesium intake and incidence of type 2 diabetes: a prospective study and meta-analysis. Arch Intern Med,167(9):956-65.

Liu J, Hanley AJG, Young TK, Harris SB, Zinman B (2006). Characteristics and prevalence of the metabolic syndrome among three ethnic groups in Canada. Int J Obes (Lond),30:669-676.

How to Cite
ZHUANPING Z, RIFANG L, QING C, SIDONG C. The Association between SIRT1 Genetic Variation and Type 2 Diabetes Mellitus Is Influenced by Dietary Intake in Elderly Chi-nese. IJPH. 47(9):1271-9.
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