Detection of Pseudocyst Forms of Trichomonas muris in Rodents from Iran
AbstractBackground: Trichomonas muris is one of the most common protozoa diagnosed in rodents. The trichomonads are generally described as presenting only trophozoite form while pseudocyst is another morphological form of trichomonads identified among gastrointestinal and genitourinary trichomonads. We identified and described different shapes of T. muris pseudocysts and trophozoite in stool samples were collected from rodents including Merinos persicus, Mus musculus and Cricetulus migratorius.Methods: In this cross-sectional study, stool samples from 204 trapped rodents were collected from Meshkin Shahr during Mar to Dec 2014. Samples were preserved in formalin 10% and PVA solution and transferred to Department of Medical Protozoology and Mycology, School of Public Health, Tehran University of Medical Sciences. Formalin-ether concentration method was used for the samples. The slides were stained with trichrome staining method and observed under light microscope.Results: The trophozoites were classified as T. muris based on size (18 to 24 µm), presence of three anterior flagella, recurrent flagellum, undulating membrane, and axostyle in direct examination and stained slides with trichrome staining method. Fifty-five out of 204 (27%) rodents were infected with T. muris in which 51(25%) samples pseudocysts form were observed. The spherical bodies of pseudocyst with almost 8 µm size, contained internalized flagella, an undulating membrane with recurrent flagellum, axostyle, and costa were seen. The pseudocysts were more prevalent than trophozoite form and pseudocysts were found with different shapes in this study.Conclusion: T. muris pseudocysts were found in stool samples of caught rodents for the first time in northwestern Iran.
Mattern C F, DANIEL WA (1980). Tritrichomonas muris in the hamster: pseudocysts and the infection of newborn. J Protozool, 27(4): 435-9.
Roach PD, Wallis PM, Olson ME (1988). The use of metronidazole, tinidazole and dimetridazole in eliminating trichomonads from laboratory mice. Lab Anim,22(4):361-4.
Lipman NS, Lampen N, Nguyen HT (1999). Identification of pseudocysts of Tritrichomonas muris in Armenian hamsters and their transmission to mice. Comp Med, 49(3):313-5.
Granger BL, Warwood SJ, Benchimol M, De Souza W (2000). Transient invagination of flagella by Tritrichomonas foetus. Parasitol Res, 86(9):699-709.
Tasca T, De Carli GA (2007). Morphological study of Tetratrichomonas didelphidis isolated from opossum Lutreolina crassicaudata by scanning electron microscopy. Parasitol Res, 100(6): 1385-8.
Kashiwagi A, Kurosaki H, Luo H et al (2009). Effects of Tritrichomonas muris on the mouse intestine: a proteomic analysis. Exp Anim, 58(5): 537-42.
Boggild AK, Sundermann CA, Estridge BH (2002). Localization of post-translationally modified α-tubulin and pseudocyst formation in tritrichomonads. Parasitol Res, 88(5): 468-74.
Mohebali M, Zarei Z, Khanaliha K et al (2017). Natural Intestinal Protozoa in Rodents (Rodentia: Gerbillinae, Murinae, Cricetinae) in Northwestern Iran. Iran J Parasitol, 12(3), 382-8.
Zarei Z, Mohebali M, Heidari Z et al (2016). Helminth Infections of Meriones persicus (Persian Jird), Mus musculus (House Mice) and Cricetulus migratorius (Grey Hamster): A Cross-Sectional Study in Meshkin-Shahr District, Northwest Iran. Iran J Parasitol , 11(2): 213-220.
Anthony NM, Ribic CA, Bautz R, Garland T (2005). Comparative effectiveness of Longworth and Sherman live traps. Wildl Soc Bull, 33(3): 1018-1026.
Noor Azian MY, San YM, Gan CC et al (2007). Prevalence of intestinal protozoa in an aborigine community in Pahang, Malaysia. Trop Biomed, 24(1): 55-62.
Pereira-Neves A, Campero CM, Martínez A, Benchimol M (2011). Identification of Tritrichomonas foetus pseudocysts in fresh preputial secretion samples from bulls. Vet Parasitol, 175(1-2):1-8.
Stachan R, Kunstýř I (1983). Minimal infectious doses and prepatent periods in Giardia muris, Spironucleus muris and Tritrichomonas muris. Zentralbl Bakteriol Mikrobiol Hyg A, 256(2):249–56.
Koyama T, Endo T, Asahi H, Kuroki T (1987). Life cycle of Tritrichomonas muris. Zentralbl Bakteriol Mikrobiol Hyg A, 264(3-4):478-86.
Pereira-Neves A, Ribeiro KC, Benchimol M (2003). Pseudocysts in trichomonads–new insights. Protist, 154(3-4):313-29.
Stachan R, Nicol C, Kunstýř I (1984). Heterogeneity of Tritrichomonas muris pseudocysts. Protist, 20(2): 157-63.
Wenrich DH (1939). The morphology of Trichomonas vaginalis, Vol Jub S Yoshida Osaka, 2: 65-76.
Samuels R (1959). Studies of Tritrichomonas batrachorum 3. Abnormal mitosis and morphogenesis. Trans Am Microsc Soc, 78(1): 49-65.
Arroyo R, Cárdenas-Guerra RE, Figueroa-Angulo EE et al (2015). Trichomonas vaginalis cysteine proteinases: Iron response in gene expression and proteolytic activity. Biomed Res Int, 2015:946787
Castro C, Menna-Barreto RFS, Fernandes NDS et al (2016). Iron-modulated pseudocyst formation in Tritrichomonas foetus. Parasitology,143(8):1034-42.
Mariante RM, Guimarães CA, Linden R, Benchimol M (2003). Hydrogen peroxide induces caspase activation and programmed cell death in the amitochondrial Tritrichomonas foetus. Histochem Cell Biol, 120: 129-41.
Afzan MY, Suresh K (2012). Pseudocyst forms of Trichomonas vaginalis from cervical neoplasia. Parasitol Res, 111(1): 371-381.
Kissinger P (2015). Trichomonas vaginalis: a review of epidemiologic, clinical and treatment issues. BMC Infect Dis,15:307.
Hernández HM, Marcet R, Sarracent J (2014). Biological roles of cysteine proteinases in the pathogenesis of Trichomonas vaginalis. Parasite, 21:54.
Hussein E, Atwa M (2008). Infectivity of Trichomonas vaginalis pseudocysts inoculated intra-vaginally in mice. J Egypt Soc Parasitol, 38(3): 749-62.