Y-chromosomal Status of Six Indo-European-Speaking Arab Subpopulations in Chaharmahal and Bakhtiari Province, Iran
Background: We analyzed the Y-chromosome haplogroups of six documented Arab subpopulations that accommodated separately in different counties of Chaharmahal and Bakhtiari Province but nowadays speak Indo-European language (Luri and Farsi).
Methods: This was an outcome study conducted in 2015 to test whether there was any genetic relatedness among some Indo-European-speaking Arab subpopulation accommodated in a geographically similar region, Chaharmahal and Bakhtiari Province, Iran. Seven main Y-chromosome bi-allelic markers were genotyped in six documented Arab subpopulations. Therefore, after DNA extraction from blood samples, PCR reaction carried out by designed primers for J1-M267, J2-M172, and J-M304, I-M170, IJ-M429, F-M89 and K-M9 markers. Then PCR products after quality control on agarose gel were sequenced.
Results: Most subjects (83.3%) belonged to F-M89 haplogroup. These subjects belonged to K-M9 (40%), J2-M172 (40%) and I-M170 (20%). Generally, there were at least three genetically distinct ancestors with a divergence date of about 22200 yr for I, 429000 for J and 47400 before present for K haplogroup and may show separate historical migrations of studied populations. As the most recent common ancestor (MRCA) of most of these populations, haplogroup F, lived about 40000-50000 yr ago, the data do not support a nearly close genetic relationship among all of these populations. However, there were populations with same haplogroups J2 (n=2), K (n=2), or with a closer MRCA, IJ haplogroups, among I and J2 haplogroups. Finding haplogroup I, a specific European haplogroup, among Arab populations was not expected.
Conclusion: Identification of various haplogroups in Arab subpopulations despite its small area and geographically conserved region of this part of Iranian plateau was unexpected.
Stoneking M (2005). Gene geographie und Sprache. In: Gene Sprachen und ihre Evolution. Ed, Hauksa, Regensburg Unversit atsverlag Regensburg GmbH. Germany, pp. 103-107.
Nasidze I, Quinque D, Rahmani M, Alemohamad SA, Stoneking M (2008). Close Genetic Relationship Between Semitic-speaking and Indo-European-speaking Groups in Iran. Ann Hum Genet,72(pt2):241–252.
Kutanan W, Kampuansai J, Fuselli S, Nakbunlung S, Seielstad M, Bertorelle G, Kangwanpong D (2011). Genetic structure of the Mon-Khmer speaking groups and their affinity to the neighbouring Tai populations in Northern Thailand. BMC Genet, 12:56.
Veeramah KR, Connell BA, Ansari Pour N et al (2010). Little genetic differentiation as assessed by uniparental markers in the presence of substantial language variation in peoples of the Cross River region of Nigeria. BMC Evol Biol, 10:92.
Sarno S, Tofanelli S, De Fanti S et al (2016). Shared language, diverging genetic histories: high-resolution analysis of Y-chromosome variability in Calabrian and Sicilian Arbereshe. Eur J Hum Genet, 24(4):600-6.
Andonian L, Rezaie S, Margaryan A et al (2011). Iranian Azeri's Y-Chromosomal Diversity in the Context of Turkish-Speaking Populations of the Middle East. Iran J Public Health, 40(1):119-23.
Gomes SM, van Oven M, Souto L et al (2017). Lack of gene-language correlation due to reciprocal female but directional male admixture in Austronesians and non-Austronesians of East Timor. Eur J Hum Genet, 25(2):246-252.
Alves I, Coelho M, Gignoux C, Damasceno A, Prista A, Rocha J (2011). Genetic homogeneity across Bantu-speaking groups from Mozambique and Angola challenges early split scenarios between East and West Bantu populations. Hum Biol, 83(1):13-38.
Karafet TM, Mendez FL, Meilerman MB, Underhill PA, Zegura SL, Hammer MF (2008). New binary polymorphisms reshape and increase resolution of the human Y chromosomal haplogroup tree. Genome Res, 18:830-838.
Semino O, Magri C, Benuzzi et al (2004). Origin diffusion and differentiation of Y-Chromosome haplogroups E and J: Inferences on the neolithization of Europe and later migratory events in the Mediterranean area. Am J Hum Genet, 74(5):1023-1034.
Chiaroni J, Underhill P, Cavalli-Sforza LL (2009). Y chromosome diversity human expansion drift and cultural evolution. Proc Natl Acad Sci, 106(48):20174-20179.
Athey TW (2005). Pitfalls in Determinations of Y Haplogroup F. J Genetic Geneal, 1:35-39.
Grugni V, Battaglia V, Hooshiar Kashani B et al (2012). Ancient migratory events in the Middle East: New clues from the Y-chromosome variation of modern Iranians. PLoS One, 7(7): e41252.
Faghih-Bahrololum M (2010). Mazarat Chahar-Mahal and Bakhtiari. Vosugh Publications.
Al-Zahery N, Pala M, Battaglia V et al (2011). In search of the genetic footprints of Sumerians: a survey of Y-chromosome and mtDNA variation in the Marsh Arabs of Iraq. BMC evol biol, 11:288.
Abu-Amero KK, Hellani A, González AM, Larruga JM, Cabrera VM, Underhill PA (2009). Saudi Arabian Y-Chromosome diversity and its relationship with nearby regions. BMC Genet, 10:59.
Rootsi S, Magri C, Kivisild T et al (2004). Phylogeography of Y-chromosome haplogroup I reveals distinct domains of prehistoric gene flow in Europe. Am J Hum Genet, 75(1):128-137.
Underhill PA, Kivisild T (2007). Use of Y chromosome and mitochondrial DNA population structure in tracing human migrations. Annu Rev Genet, 41:539–564.