Emerging Intestinal Microsporidia Infection in General Population in Jiroft District, Southeastern Iran: A Cross-sectional Study in 2013-2014
AbstractBackground: Microsporidia have been reported as the cause of opportunistic infections in immunocompromised patients in Iran and other countries. There is no data on prevalence of intestinal microsporidia in healthy population of Iran. This study aimed to provide preliminary data on the present status of microsporidia infection in the local healthy population in Jiroft, Kerman Province from southeastern Iran in 2013-2014.Methods: Fresh stool samples were randomly collected from 418 residents in rural 209 (50%) and urban 209 (50%) areas of Jiroft. All of the collected samples were concentrated with conventional formalin-ether, stained with Ryan blue. Microscopic examination was performed with high magnification on each sample separately for the demonstration of microsporidia spores. Results: Microsporidial spores were identified in 41 out of 418 (9.8%) samples including 16.41(39%) from rural areas and 25.41(61%) from urban areas. In general, there was no significant difference between sex, age, job, education, and contact with soil and livestock, water supply, gastrointestinal disorders and microsporidia infection among general population in Jiroft. Conclusion: Intestinal microsporidia infection without clinical manifestations is prevalent in general population resident in southeastern Iran. Appropriate molecular methods are needed for microsporidia species identification.
Sak B, Kvac M, Kucerova Z, Kvetonova D, Saková K (2011). Latent microsporidial infection in immunocompetent individuals-a longitudinal study. PLoS Negl Trop Dis, 5(5):e1162.
Heinz E, Williams TA, Nakjang S et al (2012). The genome of the obligate intra-cellular parasite Trachipleistophora hominis: New insights into microsporidian ge-nome dynamics and reductive evolution. PLoS Pathog, 8(10): e1002979.
Corradi N, Selman M (2013). Latest pro-gress in microsporidian genome research. J Eukaryot Microbiol, 60(3):309-12.
Hocevar SN, Paddock CD, Spak CW et al (2014). Microsporidiosis acquired through solid organ transplantation: a public health investigation. Ann Intern Med, 160(4):213-20.
Didier ES, Weiss LM (2011). Microsporidi-osis: not just in AIDS patients. Curr Opin Infect Dis, 24(5):490-5.
Lono A, Kumar S, Chye TT (2011). Detec-tion of microsporidia in local HIV- population in Malaysia. Trans R Soc Trop Med Hyg, 105(7):409-13.
Anane S, Attouchi H (2010). Microsporidio-sis: epidemiology, clinical data and thera-py. Gastroenterol Clin Biol, 34(8-9):450-64.
Loignon M, Labrecque LG, Bard C et al (2014). Cerebral microsporidiosis mani-festing as progressive multifocal leu-koencephalopathy in an HIV-infected in-dividual-a case report. AIDS Res Ther, 11:20.
Sak B, Brady D, Pelika nova M et al (2011). Unapparent microsporidial infection among immunocompetent humans in the Czech Republic. J Clin Microbiol, 49(3): 1064–1070.
Sak B, Kucerova Z, Kvac M et al (2010). Se-ropositivity for Enterocytozoon bieneusi, Czech Republic. Emerg Infect Dis, 16(2):335-7.
Mumcuoglu I, Cetin F, Dogruman Al F et al (2016). Prevalence of microsporidia in healthy individuals and immunocompe-tent patients with acute and chronic diar-rhea. Infect Dis (Lond), 48(2):133-7.
Khanaliha K, Mirjalali H, Mohebali M, Tarighi F, Rezaeian M (2014). Compari-son of Three Staining Methods for the Detection of Intestinal Microspora Spp. Iran J Parasitol, 9(4): 445–451.
Agholi M, Hatam GR, Motazedian MH (2013). HIV/AIDS-associated opportun-istic protozoal diarrhea. AIDS Res Hum Retroviruses, 29(1): 35–41.
Lobo ML, Xiao L, Antunes F, Matos O (2012). Microsporidia as emerging patho-gens and the implication for public health: A 10-year study on HIV-positive and -negative patients. Int J Parasitol, 42(2):197-205.
Ojuromi OT, Izquierdo F, Fenoy S et al (2012). Identification and characterization of microsporidia from fecal samples of HIV-positive patients from Lagos, Nige-ria. PLoS One; 7(4):e35239.
Bednarska M, Bajer A, Siński E et al (2014). Occurrence of intestinal microsporidia in immunodeficient patients in Poland. Ann Agric Environ Med, 21(2):244-8.
Meredith AL, Cleaveland SC, Brown J et al (2015). Seroprevalence of Encephalitozoon cuniculi in wild rodents, foxes and domes-tic cats in three sites in the United King-dom. Transbound Emerg Dis, 62(2):148-56.
Pan Y, Wang S, Liu X et al (2015). Seroprev-alence of Encephalitozoon cuniculi in Humans and Rabbits in China. Iran J Parasitol, 10(2):290-5.
Mirjalali H, Mohebali M, Mirhendi H et al (2014). Emerging Intestinal Microsporidia Infection in HIV+/AIDS Patients in Iran: Microscopic and Molecular Detec-tion. Iran J Parasitol, 9(2):149-54.
Khanaliha K, Mohebali M, Davoudi S et al (2015). Detection of Emergence Cyclospora cayetanensis in A HIV+/AIDS Patient with Diarrhea from Tehran: A Case Report. Iran J Public Health, 44(6): 865–868.
Agholi M, Hatam GR, Motazedian MH (2013). Microsporidia and coccidia as causes of persistence diarrhea among liv-er transplant children: incidence rate and species/genotypes. Pediatr Infect Dis J, 32(2):185-7.
Ryan NJ, Sutherland G, Coughlan K et al (1993). A new trichrome-blue stain for detection of microsporidial species in urine, stool, and nasopharyngeal speci-mens. J Clin Microbiol, 31(12):3264-9.
Didier E, Stovall M, Green L, Brindley P, Sestak K, Didier P (2004). Epidemiology of microsporidiosis: sources and modes of transmission. Vet Parasitol, 126(1-2):145-66.
Conteas CN, Didier ES, Berlin OG (1997). Workup of gastrointestinal microsporidi-osis. Dig Dis, 15(6):330-45.
Gumbo T, Sarbah S, Gangaidzo IT et al (1999). Intestinal parasites in patients with diarrhea and human immunodeficiency virus infection in Zimbabwe. AIDS, 13(7):819-21.
Beatriz L, Isabel L-M, Cristina A et al (2002). Intestinal microsporidiosis due to Entero-cytozoon bieneusi in elderly human immuno-deficiency virus–negative patients from Vigo, Spain. Clin Infect Dis, 34(7):918-21.
Nkinin SW, Asonganyi T, Didier ES, Kaneshiro ES et al (2007). Microsporidi-an infection is prevalent in healthy people in Cameroon. J Clin Microbiol, 45(9): 2841–2846.
Tumwine JK, Kekitiinwa A, Bakeera-Kitaka S et al (2005). Cryptosporidiosis and mi-crosporidiosis in Ugandan children with persistent diarrhea with and without con-current infection with the human immu-nodeficiency virus. Am J Trop Med Hyg, 73(5):921-5.
Tumwine JK, Kekitiinwa A, Nabukeera N et al (2002). Enterocytozoon bieneusi among children with diarrhea attending Mulago Hospital in Uganda. Am J Trop Med Hyg, 67(3):299-303.
Halánová M, Valencakova A, Kvac M et al (2013). Occurrence of microsporidia as emerging pathogens in Slovak Roma children and their impact on public health. Ann Agric Environ Med, 20(4):695-8.
Coyle CM, Wittner M, Kotler DP et al (1996). Prevalence of microsporidiosis due to Enterocytozoon bieneusi and Encepha-litozoon (Septata) intestinalis among patients with AIDS-related diarrhea: determina-tion by polymerase chain reaction to the microsporidian small-subunit rRNA gene. Clin Infect Dis, 23(5):1002-6.